Catalog Number - 65518-10P

Zx Similars C18™ is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octadecyl (C18) bonded phase. Designed as a cost-effective alternative to Agilent Zorbax® C18 columns, it offers comparable chromatographic behavior for USP L1 methods while providing the strong hydrophobic retention, reproducibility, and broad applicability expected from a conventional C18 stationary phase.

65518-10P is a 100 mm x 4.6 mm ID analytical column packed with 5 µm Zx Similars C18 particles. The 100 mm length provides an excellent balance between analysis speed and chromatographic resolution, making it suitable for routine analytical methods, quality control testing, and method development. The 5 µm particle size provides excellent robustness, broad method compatibility, and lower operating pressure than smaller particle columns, making it well suited for conventional HPLC systems. Typical operating flow rates are approximately 1.0–2.0 mL/min.

This USP L1 column is ideal for hydrophobic and neutral compounds requiring strong reversed-phase retention. Common applications include pharmaceutical analysis, long-chain fatty acid separations, quality control testing, and method transfer from Agilent Zorbax® C18 columns and similar USP L1 methods.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L1 methods

• Comparable selectivity to Agilent Zorbax® C18 columns

• Balanced speed and resolution with 100 mm length

• Broad compatibility with conventional HPLC systems

• Lower operating pressure than smaller particle columns

• Strong hydrophobic retention

• End-capped C18 bonded phase

Typical Applications

• USP L1 methods

• Reversed-phase HPLC

• Pharmaceutical analysis

• Quality control testing

• Method development

• Long-chain fatty acid analysis

• Routine analytical separations

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Zx Similars C18

• USP Classification: L1

• Particle Size: 5 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C18 (Octadecyl)

• Endcapped: Yes

• Column Dimensions: 4.6 mm ID x 100 mm

• Length: 100 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• 100 mm length balances speed and resolution

• Compatible with conventional HPLC systems

• Lower operating pressure than smaller particle columns

• Excellent alternative for many USP L1 methods

• Strong retention for hydrophobic compounds

• Suitable for routine analytical workflows

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